WINETECH Technical Yearbook 2021

Location

Site

GPS co-ordinates

* Fungal isolate

Isolation method

Fungal species

** Mortality ± SE

­

8 9

18.411317° - 34.045568° 18.404093° - 34.034385°

Metarhizium robertsii Clonostachys rosea

46.0 ± 1.0 52.0 ± 1.0 44.0 ± 1.0 18.0 ± 1.1

CPUT19

I

SM8 SMB SM9

S

Fusarium solani

10 11 12 13 14 15 16 17 18 19 20 21 22

19.320406° - 34.059153° 19.320306° - 34.062953° 19.002866° - 34.720840° 19.117831° - 34.918309° 18.958800° - 33.766000° 20.014027° - 33.988441° 19.987705° - 33.991239° 19.987805° - 33.991239° 20.127851° - 33.887736° 20.127851° - 33.887736° 19.117514° - 33.991239° 18.413525° - 34.015845° 19.167682°- 34.551830°

I

Clonostachys rosea

S

­ ­

Paarl

Clonostachys rosea Beauveria bassiana Clonostachys rosea

50.0 ± 2.0 82.0 ± 2.0 32.0 ± 2.0

SM10 SM13

I I

Malmesbury

CPUT18

S

­

Fusarium solani

56.0 ± 1.0

SM1

S

­ ­ ­ ­

Franschhoek

Aspergillus laciniosus Aspergillus oryzae Metarhizium robertsii

SM4B SM12

S

I I

31.0 ± 6.0

CPUT22

For more information, contact Kwaku Achiano at AchianoK@arc.agric.za.

SUMMARY

isolated with methods of insect baiting and selective media. In addition, fungal isolates were tested against a key grapevine pest, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae). Pathogenicity of fungal

strains against P. ficus was assessed with an immersion bioassay at a concentration of 19 108 conidia mL –1 . Strains of Beauvaria bassiana (Hypocreales: Cordycipitaceae) caused the highest mortalities.

Soil samples collected from vineyards in the Western Cape were examined for the presence of EPF prevalence. Fungi were

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