South Africa Wine Research Projects

2. VITICULTURE RESEARCH NEW PROJECT

ESTABLISHMENT OF GRAPEVINE LEAFROLL ASSOCIATED VIRUS 1 AND 2 ELISAS

PATHSOL GP 24-02 Prof Gerhard Pietersen, Patho Solutions Project is co-funded by Patho Solutions Start date: 2024

AIM AND INDUSTRY RELEVANCE: Grapevine Leafroll-associated Virus-3 (GLRaV-3) is the most widespread and the focus of control of leafroll-associated viruses. Other associated viruses like GLRaV-1 and -2 also occur; however, only GLRaV-3 ELISA tests are available, so there is a need for ELISAs for GLRaV-1 and -2. The Vine Improvement Association (VIA) certification scheme mandates GLRaV-1, -2, and -3 tests. In the past, this was done via a locally prepared single ELISA system that could detect these three viruses. This test, however, is no longer available, and currently, only GLRaV-3 testing is locally available and affordable. This project aims to develop ELISA tests to detect GLRaV-1 and GLRaV-2 and, if successful, combine them into a GLRaV1, -2, & -3 cocktail ELISA, which can detect all three viruses within a single test.

SOUTH AFRICA WINE RESEARCH 2024

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